Other

Part:BBa_I716334:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-07-11)

ThpD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
    Illegal XhoI site found at 906
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 465
    Illegal SapI site found at 704


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

ThpD
From IGEM07
PCR ThpD F/R on ThpD (916bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BglII/XhoI, 2054+910 L)
Product is pBca9145-BBa I716334



ThpDF Forwar BglII site anneals to ThpD cggatAGATCTatgaccaccgaagtacgcgc

ThpDR Reverse XhoI site anneals to ThpD gtcagctcgagctacttcaccggggtgaagt


References